Experiment summary for ENCSR882DWM

doi:10.17989/ENCSR882DWM

Summary

Status
released
Assay
PRO-cap
Biosample summary
Homo sapiens K562
Biosample Type
cell line
Replication type
isogenic
Description
CoPRO experiment. A run-on assay was performed on captured nascent RNA with cap state selection. In this experiment, only uncapped nascent RNA was selected and no PCR amplification was done.
Nucleic acid type
RNA
Average fragment size
78 [1-2]67 [1-1]74 [2-2]72 [2-1]
Size selection method
Streptavidin magnetic beads
Library construction method
PCR-free, uncapped RNA enrichment
Strand specificity
Strand-specific (reverse)

Attribution

ENCODE4 project
Lab
John Lis, Cornell
Award
UM1HG009393 (John Lis, Cornell)
Project
ENCODE
Aliases
john-lis:copro-experiment-Uncapped-PCRFree
Date submitted
November 19, 2021
Date released
December 14, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
12Homo sapiens K562 cell lineENCBS154MMUENCLB264LBO
11Homo sapiens K562 cell lineENCBS154MMUENCLB465HHU
22Homo sapiens K562 cell lineENCBS800REPENCLB049AQJ
21Homo sapiens K562 cell lineENCBS800REPENCLB290TVD