Experiment summary for ENCSR879BNI
doi:10.17989/ENCSR879BNI
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Mus musculus strain C57BL/6J macrophage originated from bone marrow treated with 100 ng/mL lipoprotein A for 30 minutes
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- mRNA-seq on whole cell extract RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for30 minutes.
- Treatments
- 100 ng/mL lipoprotein A for 30 minutes
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 1µg
- Size range
- 250-400
- Fragmentation methods
- see document
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 4000
Attribution
Genomics of Gene Regulation Project
- Lab
- Alexander Hoffmann, UCLA
- Award
- U01HG007912 (Alexander Hoffmann, UCLA)
- Project
- GGR
- External resources
- Aliases
- alexander-hoffmann:PolyA.Naive.030.exp
- Date submitted
- January 20, 2017
- Date released
- November 20, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6J macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 30 minutes | ENCBS275UTU | ENCLB950NJI |
| 2 | 1 | Mus musculus strain C57BL/6J macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 30 minutes | ENCBS475IIX | ENCLB009AQC |