Experiment summary for ENCSR864KVZ

doi:10.17989/ENCSR864KVZ

Summary

Status
released
Assay
ChIP-seq (Histone ChIP-seq)
Target
H3K27ac
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 20 minutes
Biosample Type
cell line
Replication type
isogenic
Description
H3K27ac ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 20 mins.
Treatments
100 nM dexamethasone (CHEBI:41879) for 20 minutes
Nucleic acid type
DNA
Library starting quantity
7ng
Size range
>200
Fragmentation methods
sonication (Bioruptor Twin)
Size selection method
dual SPRI
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:H3K27ac.t20m
Date submitted
October 13, 2017
Date released
October 19, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
21Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutesENCBS331TBMENCAB037IXKENCLB603HPF
11Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutesENCBS010CCHENCAB037IXKENCLB756VWU
31Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutesENCBS026DZZENCAB037IXKENCLB127NXZ