Experiment summary for ENCSR862DMN

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP396 (unc-119(tm4063) III; wgIs396) whole organism L4 larva (53 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing hlh-11-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:HLH-11_OP396_L4
Date submitted
May 31, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP396 (unc-119(tm4063) III; wgIs396) mixed sex L4 larva (53 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-hlh-11ENCBS166EMAENCGM239EYEENCAB728YTOENCLB403ZAO
21strain OP396 (unc-119(tm4063) III; wgIs396) mixed sex L4 larva (53 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-hlh-11ENCBS986WRPENCGM239EYEENCAB728YTOENCLB830SJO