Experiment summary for ENCSR847UWT
doi:10.17989/ENCSR847UWT
Summary
- Status
- released
- Assay
- HiC (in situ Hi-C)
- Biosample summary
- Mus musculus strain C57BL/6J mature B cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- in situ MboI Hi-C
- Treatments
- 50 μg/mL lipopolysaccharide (CHEBI:16412) for 24 hours, 5 ng/mL Interleukin-4 (UniProtKB:P05112) for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin (CHEBI:25675) for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose (CHEBI:15866) for 2 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 5000000cells
- Size range
- 300-500
- Fragmentation methods
- chemical (MboI restriction)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq X Ten, Illumina HiSeq 2500
Attribution
ENCODE4 project
- Lab
- Erez Aiden, Baylor
- Award
- UM1HG009375 (Erez Aiden, Baylor)
- Project
- ENCODE
- External resources
- Aliases
- erez-aiden:mouse-activated-B-cells-oligo-24hours
- Date submitted
- November 1, 2018
- Date released
- January 28, 2019
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 3 | 3 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS759PYV | ENCLB673XSW |
| 3 | 1 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS759PYV | ENCLB919BEN |
| 1 | 4 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS945UNI | ENCLB535IAP |
| 3 | 4 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS759PYV | ENCLB813JST |
| 1 | 1 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS945UNI | ENCLB949CMR |
| 2 | 2 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS351ZCF | ENCLB861VIW |
| 3 | 2 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS759PYV | ENCLB260LQS |
| 1 | 5 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS945UNI | ENCLB194FYB |
| 1 | 2 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS945UNI | ENCLB999CQE |
| 2 | 1 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS351ZCF | ENCLB511JAA |
| 1 | 3 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS945UNI | ENCLB586COD |
| 2 | 4 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS351ZCF | ENCLB565DLH |
| 4 | 1 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS110BFP | ENCLB489BXY |
| 2 | 3 | Mus musculus strain C57BL/6J mature B primary cell treated with 50 μg/mL lipopolysaccharide for 24 hours, 5 ng/mL Interleukin-4 for 24 hours, 500 ng/mL anti-CD180 for 24 hours, 99.7 ng/mL oligomycin for 2 hours, 820.8 μg/mL 2-Deoxy-D-glucose for 2 hours | ENCBS351ZCF | ENCLB141YNO |