Experiment summary for ENCSR845MNM

doi:10.17989/ENCSR845MNM

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
SRSF7
Biosample summary
Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SRSF7
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with a CRISPR gRNA against SRSF7. (SRSF7_BGHcLV03)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10 [1-1]9.9 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:SRSF7_BGHcLV03
Date submitted
March 9, 2016
Date released
March 17, 2016
Gene Silencing Series
ENCSR842FAV 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF7ENCBS111IHCENCGM146LLTENCLB708RGH
21Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF7ENCBS670FFGENCGM146LLTENCLB734HEX