Experiment summary for ENCSR844VCY

doi:10.17989/ENCSR844VCY

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
ceh-48
Biosample summary
Caenorhabditis elegans strain OP631 (unc-119(tm4063) III; wgIs631) whole organism late embryo 3.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting celegans ceh-48
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing CEH-48-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CEH-48-OP631-LE
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF504VHQ, ENCFF074CND were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain OP631 (unc-119(tm4063) III; wgIs631) mixed sex late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting celegans ceh-48ENCBS875OLTENCGM943SSAENCAB728YTOENCLB798MKY
11strain OP631 (unc-119(tm4063) III; wgIs631) mixed sex late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting celegans ceh-48ENCBS468IPPENCGM943SSAENCAB728YTOENCLB857PQN