Experiment summary for ENCSR843RGD

Summary

Status
released
Assay
single cell isolation followed by RNA-seq (scRNA-seq)
Biosample summary
Mus musculus C57BL/6 forelimb embryo (15.5 days)
Biosample Type
single cell
Replication type
unreplicated
Description
C1 single cell e15.5 mouse forelimb. Putative perichondrial cell
Nucleic acid type
RNA
Library starting quantity
1cells
Size range
300-500
Lysis method
Clontech UltraLow for Illumina sequencing
Extraction method
C1 fluidigm
Fragmentation methods
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Spike-ins datasets
ENCSR535LMC
Library construction platform
Fluidigm C1 System

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Aliases
barbara-wold:c1_e15.5_mouse_limb_run14_20048_E6
Date released
February 12, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11strain B6NCrl embryo (15.5 days) forelimb single cellENCBS045HQKENCLB270EPQ