Experiment summary for ENCSR842NDO
doi:10.17989/ENCSR842NDO
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Homo sapiens natural killer cell female adult (41 years) treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-15 for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, 100 ng/mL Interleukin-18 for 72 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- h.CD56+_DONOR 3: 72 hour activation IL-12+IL-15+IL-18, 100ng/ml
- Treatments
- 100 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 72 hours, 100 ng/mL Interleukin-15 (UniProtKB:P40933) for 72 hours, 100 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 72 hours, 100 ng/mL Interleukin-18 (UniProtKB:Q14116) for 72 hours
- Nucleic acid type
- RNA
- RNA integrity number
- 9
- Library starting quantity
- 330ng
- Average fragment size
- 277 [1-2]274 [1-1]
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR597UQL
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE4 project
- Lab
- Barbara Wold, Caltech
- Award
- UM1HG009443 (Barbara Wold, Caltech)
- Project
- ENCODE
- External resources
- Date released
- July 1, 2022
- Annotation (physical modeling)
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 2 | Homo sapiens female adult (41 years) natural killer primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-15 for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, 100 ng/mL Interleukin-18 for 72 hours | ENCBS734EVS | ENCLB715DGZ |
| 1 | 1 | Homo sapiens female adult (41 years) natural killer primary cell treated with 100 ng/mL Interleukin-12 subunit alpha for 72 hours, 100 ng/mL Interleukin-15 for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours, 100 ng/mL Interleukin-18 for 72 hours | ENCBS734EVS | ENCLB961ZIH |