Experiment summary for ENCSR840QOH

doi:10.17989/ENCSR840QOH

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
ETF1
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens ETF1
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with an shRNA knockdown against ETF1. (ETF1_BGHLV37)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.5 [1-1]9.7 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:ETF1_BGHLV37
Date submitted
December 12, 2016
Date released
January 4, 2017
Gene Silencing Series
ENCSR996VFO 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens ETF1ENCBS232YHBENCGM226GBVENCLB244VSF
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens ETF1ENCBS714ECQENCGM226GBVENCLB114ADK