Experiment summary for ENCSR826CFP
doi:10.17989/ENCSR826CFP
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- CG11762
- Biosample summary
- Drosophila melanogaster strain CG11762-GFP whole organism prepupa genetically modified (insertion) using site-specific recombination targeting D. melanogaster ouib
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing CG11762-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:CG11762-GFP
- Date submitted
- April 9, 2016
- Date released
- July 6, 2016
- Submitter comment
- ENCFF648ZMJ, ENCFF501FBO were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files. They also note that although there is a warning for insufficient read depth, it only affects replicate 3 and replicate 1 and 2 have sufficient read depth (minimum 3M usable fragments).
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ouib | ENCBS259BUH | ENCGM910LHE | ENCAB728YTO | ENCLB573RIS |
| 1 | 1 | Drosophila melanogaster strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ouib | ENCBS094IMU | ENCGM910LHE | ENCAB728YTO | ENCLB044KYI |
| 3 | 1 | Drosophila melanogaster strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ouib | ENCBS037YRZ | ENCGM910LHE | ENCAB728YTO | ENCLB195ULI |