Experiment summary for ENCSR826CFP

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Drosophila melanogaster strain CG11762-GFP whole organism prepupa genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CG11762-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CG11762-GFP
Date submitted
April 9, 2016
Date released
July 6, 2016
Submitter comment
ENCFF648ZMJ, ENCFF501FBO were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files. They also note that although there is a warning for insufficient read depth, it only affects replicate 3 and replicate 1 and 2 have sufficient read depth (minimum 3M usable fragments).

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG11762ENCBS259BUHENCGM910LHEENCAB728YTOENCLB573RIS
11strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG11762ENCBS094IMUENCGM910LHEENCAB728YTOENCLB044KYI
31strain CG11762-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG11762ENCBS037YRZENCGM910LHEENCAB728YTOENCLB195ULI