Experiment summary for ENCSR825RYR

doi:10.17989/ENCSR825RYR

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
CWC15
Biosample summary
Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens CWC15
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against CWC15. (CWC15-BGKcLV28)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.5 [1-1]9.6 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR156CIL
Controls

Attribution

ENCODE4 project
Lab
Brenton Graveley, UConn
Award
U41HG009889 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:CWC15-BGKcLV28
Date released
November 19, 2021
Gene Silencing Series
ENCSR082GWT 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens CWC15ENCBS757ZRTENCGM015SGIENCLB377VVP
21Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens CWC15ENCBS922IOXENCGM015SGIENCLB130FIV