Experiment summary for ENCSR823LPZ
doi:10.17989/ENCSR823LPZ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- sptf-1
- Biosample summary
- Caenorhabditis elegans strain OP707 (unc-119(tm4063) III; wgIs707) whole organism mixed stage (embryo) 2.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans sptf-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing SPTF-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:SPTF-1-OP707-EM
- Date submitted
- October 6, 2017
- Date released
- November 6, 2017
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP707 (unc-119(tm4063) III; wgIs707) mixed sex mixed stage (embryo) 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sptf-1 | ENCBS828VGL | ENCGM921XCT | ENCAB728YTO | ENCLB702LAI |
| 2 | 1 | Caenorhabditis elegans strain OP707 (unc-119(tm4063) III; wgIs707) mixed sex mixed stage (embryo) 2.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sptf-1 | ENCBS463FKU | ENCGM921XCT | ENCAB728YTO | ENCLB567WGR |