Experiment summary for ENCSR823IAC

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP550 (unc-119(tm4063) III; wgIs550) whole organism young adult 66 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing F57C9.4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:F57C9.4-OP550-yAd
Date submitted
October 5, 2017
Date released
November 6, 2017

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
51strain OP550 (unc-119(tm4063) III; wgIs550) mixed sex young adult 66 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-F57C9.4ENCBS362BUDENCGM521EDFENCAB728YTOENCLB124FUC
61strain OP550 (unc-119(tm4063) III; wgIs550) mixed sex young adult 66 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-F57C9.4ENCBS798IOAENCGM521EDFENCAB728YTOENCLB279TVH