Experiment summary for ENCSR822EWO
doi:10.17989/ENCSR822EWO
Summary
- Status
- released
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Control type
- control
- Biosample summary
- Mus musculus strain B6CASTF1/J cerebellar granule layer tissue male adult (14 months)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- LCMD-503,504_Mm_Castaneus_x_C57Bl-granule-layer-area-dissection
- Nucleic acid type
- RNA
- Library starting quantity
- 30cell-equivalent
- Size range
- >200
- Lysis method
- Clontech UltraLow for Illumina sequencing
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ, ENCSR133ALU
- Platform
- Illumina HiSeq 2000, Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Date submitted
- June 26, 2015
- Date released
- October 13, 2015
- Organism Development Series
- ENCSR097PJJ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
2 | 1 | Mus musculus strain B6CASTF1/J male adult (14 months) cerebellar granule layer tissue | ENCBS616ZXC | ENCLB646JVH |
1 | 1 | Mus musculus strain B6CASTF1/J male adult (14 months) cerebellar granule layer tissue | ENCBS497KQR | ENCLB021ACJ |