Experiment summary for ENCSR813WNF

doi:10.17989/ENCSR813WNF

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
pag-3
Biosample summary
Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) whole organism hermaphrodite late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans pag-3
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing PAG-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:PAG-3-OP154-LE
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF868ZDG, ENCFF690JTQ, ENCFF315YYY, ENCFF287VIE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3ENCBS510JFBENCGM183DVCENCAB728YTOENCLB881EBB
21Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3ENCBS814QFXENCGM183DVCENCAB728YTOENCLB140TCE