Experiment summary for ENCSR813WNF
doi:10.17989/ENCSR813WNF
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- pag-3
- Biosample summary
- Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) whole organism hermaphrodite late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans pag-3
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing PAG-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:PAG-3-OP154-LE
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF868ZDG, ENCFF690JTQ, ENCFF315YYY, ENCFF287VIE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3 | ENCBS510JFB | ENCGM183DVC | ENCAB728YTO | ENCLB881EBB |
2 | 1 | Caenorhabditis elegans strain OP154 (unc-119(ed3) III; wgIs154) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans pag-3 | ENCBS814QFX | ENCGM183DVC | ENCAB728YTO | ENCLB140TCE |