Experiment summary for ENCSR811ZML
doi:10.17989/ENCSR811ZML
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- flh-1
- Biosample summary
- Caenorhabditis elegans strain OP702 (unc-119(tm4063); wgIs702 [flh-1::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism mixed stage (embryo) (2 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans flh-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing flh-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:FLH-1_OP702_EE
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
2 | 1 | Caenorhabditis elegans strain OP702 (unc-119(tm4063); wgIs702 [flh-1::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (2 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans flh-1 | ENCBS145WPO | ENCGM063PTZ | ENCAB728YTO | ENCLB467OEF |
1 | 1 | Caenorhabditis elegans strain OP702 (unc-119(tm4063); wgIs702 [flh-1::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (2 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans flh-1 | ENCBS237RSG | ENCGM063PTZ | ENCAB728YTO | ENCLB128KJM |