Experiment summary for ENCSR790GFX
doi:10.17989/ENCSR790GFX
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- dl
- Biosample summary
- Drosophila melanogaster strain dl-large-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster dl
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing dl-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-275
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- E-Gel
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- Aliases
- kevin-white:dl-RC-GFP
- Date submitted
- July 23, 2015
- Date released
- July 6, 2018
- Submitter comment
- The modERN consortia has concluded that the anti-GFP antibody Ab290 (Abcam) is suitable for ChIP-seq in C. elegans and D. melanogaster. ENCODE has not accepted immunofluorescence as a valid assay for antibody specificity. However, we have found that immunofluorescence on GFP tagged lines using Ab290 can recapitulate expression patterns generated by RNA in situ hybridization. Furthermore, several unpublished concordant ChIP-seq datasets have been previously generated with Ab290 and either TF specific antibodies or other ENCODE-validated anti-GFP antibodies.ENCFF261RMY, ENCFF717BTC, ENCFF862TOV, ENCFF189RUN, ENCFF349XWD and ENCFF090MRH were originally associated with this experiment but actually belong to ENCSR484YRI.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain dl-large-GFP wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dl | ENCBS882HRJ | ENCGM172SBJ | ENCAB426GGE | ENCLB587TDV |
| 3 | 1 | Drosophila melanogaster strain dl-large-GFP wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dl | ENCBS857HEM | ENCGM172SBJ | ENCAB426GGE | ENCLB317LWT |