Experiment summary for ENCSR787QXE
doi:10.17989/ENCSR787QXE
Summary
- Status
- released
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Biosample summary
- Mus musculus strain B6NCrl forelimb tissue embryo (13.5 days)
- Biosample Type
- tissue
- Replication type
- unreplicated
- Description
- 10X Genomics Chromium 3' single-cell RNA-Seq (version 2 kit) that was sequenced in PE151 format. They are from monocellular suspensions of 8,000 dispersed embryonic limb buds.
- Nucleic acid type
- polyadenylated mRNA
- Size range
- 300-600
- Strand specificity
- unstranded
- Library construction platform
- 10X Genomics Chromium Controller
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Barbara Wold, Caltech
- Award
- UM1HG009443 (Barbara Wold, Caltech)
- Project
- ENCODE
- Date submitted
- January 29, 2018
- Date released
- December 19, 2018
- Organism Development Series
- ENCSR062VQC
- Submitter comment
- Please see the attached document for an overview of the library structure and features in the data files (molecular and cellular barcodes, etc...). These data can be processed using the 10X Cell Ranger software package. All of the files come from a single library/run and can be combined in downstream analysis.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Mus musculus strain B6NCrl embryo (13.5 days) forelimb tissue | ENCBS688AVZ | ENCLB268GZV |