Experiment summary for ENCSR787QXE

doi:10.17989/ENCSR787QXE

Summary

Status
released
Assay
single-cell RNA sequencing assay (scRNA-seq)
Biosample summary
Mus musculus strain B6NCrl forelimb tissue embryo (13.5 days)
Biosample Type
tissue
Replication type
unreplicated
Description
10X Genomics Chromium 3' single-cell RNA-Seq (version 2 kit) that was sequenced in PE151 format. They are from monocellular suspensions of 8,000 dispersed embryonic limb buds.
Nucleic acid type
polyadenylated mRNA
Size range
300-600
Strand specificity
unstranded
Library construction platform
10X Genomics Chromium Controller

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Date submitted
January 29, 2018
Date released
December 19, 2018
Organism Development Series
ENCSR062VQC 
Submitter comment
Please see the attached document for an overview of the library structure and features in the data files (molecular and cellular barcodes, etc...). These data can be processed using the 10X Cell Ranger software package. All of the files come from a single library/run and can be combined in downstream analysis.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Mus musculus strain B6NCrl embryo (13.5 days) forelimb tissueENCBS688AVZENCLB268GZV