Experiment summary for ENCSR780ESQ

Summary

Status
revoked
Assay
ChIP-seq (TF ChIP-seq)
Target
KLF14
Biosample summary
Homo sapiens HEK293 genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14
Biosample Type
cell line
Replication type
isogenic
Description
eGFP-KLF14 ChIP-seq on human HEK293 cells.
Nucleic acid type
DNA
Size range
450-650

Attribution

ENCODE3 project
Lab
Michael Snyder, Stanford
Award
U54HG006996 (Michael Snyder, Stanford)
Project
ENCODE
External resources
Aliases
michael-snyder:HEK_eGFP-KLF14_FRT_ChIP
Date submitted
March 31, 2016
Date released
January 29, 2018
Submitter comment
Kundaje lab found this experiment falls in a relatively large collection of replicates across multiple ZNF TFs that are EXACTLY identical. This is definitely not co-binding TFs since the overlap and concordance is excessively high. It is also impossible to find expected motifs enriched in these peak regions. The peaks seem to be falling in AT repeat regions likely capturing some kind of eGFP ChIPing artifacts. This is confirmed visually in browser sessions.
Tags
Search for all Experiment with internal tag RegulomeDB_2_0Search for all Experiment with internal tag RegulomeDB_1_0Search for all Experiment with internal tag ENCYCLOPEDIAv5

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14ENCBS949DFIENCGM433NGKENCAB976SGGENCLB671MXW
21Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14ENCBS348BZRENCGM433NGKENCAB615WUNENCLB009GIZ