Experiment summary for ENCSR780ESQ
Summary
- Status
- revoked
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- KLF14
- Biosample summary
- Homo sapiens HEK293 genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- eGFP-KLF14 ChIP-seq on human HEK293 cells.
- Nucleic acid type
- DNA
- Size range
- 450-650
Attribution
ENCODE3 project
- Lab
- Michael Snyder, Stanford
- Award
- U54HG006996 (Michael Snyder, Stanford)
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:HEK_eGFP-KLF14_FRT_ChIP
- Date submitted
- March 31, 2016
- Date released
- January 29, 2018
- Submitter comment
- Kundaje lab found this experiment falls in a relatively large collection of replicates across multiple ZNF TFs that are EXACTLY identical. This is definitely not co-binding TFs since the overlap and concordance is excessively high. It is also impossible to find expected motifs enriched in these peak regions. The peaks seem to be falling in AT repeat regions likely capturing some kind of eGFP ChIPing artifacts. This is confirmed visually in browser sessions.
- Tags
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14 | ENCBS949DFI | ENCGM433NGK | ENCAB976SGG | ENCLB671MXW |
| 2 | 1 | Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting H. sapiens KLF14 | ENCBS348BZR | ENCGM433NGK | ENCAB615WUN | ENCLB009GIZ |