Experiment summary for ENCSR763GFW
doi:10.17989/ENCSR763GFW
Summary
- Status
- released
- Assay
- siRNA knockdown followed by RNA-seq (siRNA RNA-seq)
- Target
- snRNP-U1-70K
- Biosample summary
- Drosophila melanogaster strain Oregon-R S2 expressing RNAi targeting D. melanogaster snRNP-U1-70K
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNAi knockdown against snRNP-U1-70K in S2-DRSC cell line followed by total RNA-seq (Oligo-dT selected, double stranded cDNA).
- Nucleic acid type
- RNA
- Size range
- >200
- Extraction method
- Trizol
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR358OMW
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modENCODE Project
- Lab
- Brenton Graveley, UConn
- Award
- U01HG004271 (Susan Celniker, LBNL)
- Project
- modENCODE
- External resources
- References
- Aliases
- brenton-graveley:S2-DRSC-CG8749-RNAi
- Date submitted
- March 31, 2014
- Date released
- March 31, 2014
- Gene Silencing Series
- ENCSR808WNU
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
2 | 1 | Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster snRNP-U1-70K | ENCBS010RAQ | ENCGM924PQB | ENCLB388LGS |
1 | 1 | Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster snRNP-U1-70K | ENCBS956ORA | ENCGM924PQB | ENCLB188IAE |