Experiment summary for ENCSR758GOA

doi:10.17989/ENCSR758GOA

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
CREB5
Biosample summary
Homo sapiens SK-N-SH genetically modified (insertion) using CRISPR targeting H. sapiens CREB5 treated with 6 μM all-trans-retinoic acid for 48 hours
Biosample Type
cell line
Replication type
isogenic
Description
eGFP-CREB5 ChIP-seq on human SK-N-SH
Treatments
6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
Nucleic acid type
DNA
Size range
250-450
Fragmentation methods
sonication (Bioruptor Pico)

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award
UM1HG009442 (Michael Snyder, Stanford)
Project
ENCODE
External resources
Aliases
michael-snyder:pCS-199, michael-snyder:pCS-eGFP-CREB5 SK-H-SH ChIP
Date submitted
January 18, 2019
Date released
August 28, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB5 treated with 6 μM all-trans-retinoic acid for 48 hoursENCBS860TSUENCGM035OLJENCAB728YTOENCLB455IYY
21Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB5 treated with 6 μM all-trans-retinoic acid for 48 hoursENCBS532CTNENCGM035OLJENCAB728YTOENCLB802EGS