Experiment summary for ENCSR757OPC

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
vab-15
Biosample summary
Caenorhabditis elegans strain XIL99 whole organism L1 larva at L1 larva starved after bleaching stage genetically modified using CRISPR
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing VAB-15-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:VAB-15-XIL99-L1
Date submitted
October 5, 2017
Date released
November 6, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11strain XIL99 mixed sex L1 larva at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting vab-15ENCBS096ZAKENCGM896RUAENCAB728YTOENCLB028PZX
21strain XIL99 mixed sex L1 larva at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting vab-15ENCBS564SNSENCGM896RUAENCAB728YTOENCLB350ABJ