Experiment summary for ENCSR748ZJA

doi:10.17989/ENCSR748ZJA

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Dif
Biosample summary
Drosophila melanogaster strain Dif-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster Dif
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Dif-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-300 [4-1]200-275 [3-1]
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
E-Gel
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Dif-GFP
Date submitted
July 23, 2015
Date released
July 6, 2018
Submitter comment
The modERN consortia has concluded that the anti-GFP antibody Ab290 (Abcam) is suitable for ChIP-seq in C. elegans and D. melanogaster. ENCODE has not accepted immunofluorescence as a valid assay for antibody specificity. However, we have found that immunofluorescence on GFP tagged lines using Ab290 can recapitulate expression patterns generated by RNA in situ hybridization. Furthermore, several unpublished concordant ChIP-seq datasets have been previously generated with Ab290 and either TF specific antibodies or other ENCODE-validated anti-GFP antibodies.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain Dif-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster DifENCBS973ACZENCGM515WNZENCAB426GGEENCLB712WGL
31Drosophila melanogaster strain Dif-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster DifENCBS121VAQENCGM515WNZENCAB426GGEENCLB639PET