Experiment summary for ENCSR748ZJA
doi:10.17989/ENCSR748ZJA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Dif
- Biosample summary
- Drosophila melanogaster strain Dif-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster Dif
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Dif-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-300 [4-1]200-275 [3-1]
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- E-Gel
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Dif-GFP
- Date submitted
- July 23, 2015
- Date released
- July 6, 2018
- Submitter comment
- The modERN consortia has concluded that the anti-GFP antibody Ab290 (Abcam) is suitable for ChIP-seq in C. elegans and D. melanogaster. ENCODE has not accepted immunofluorescence as a valid assay for antibody specificity. However, we have found that immunofluorescence on GFP tagged lines using Ab290 can recapitulate expression patterns generated by RNA in situ hybridization. Furthermore, several unpublished concordant ChIP-seq datasets have been previously generated with Ab290 and either TF specific antibodies or other ENCODE-validated anti-GFP antibodies.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
4 | 1 | Drosophila melanogaster strain Dif-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Dif | ENCBS973ACZ | ENCGM515WNZ | ENCAB426GGE | ENCLB712WGL |
3 | 1 | Drosophila melanogaster strain Dif-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Dif | ENCBS121VAQ | ENCGM515WNZ | ENCAB426GGE | ENCLB639PET |