Experiment summary for ENCSR733DAS
Summary
- Status
- released
- Assay
- PRO-cap
- Biosample summary
- Homo sapiens HEK293T genetically modified (insertion) using CRISPR targeting H. sapiens ZNF143 treated with 500 nM dTAGV-1 for 6 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- A run-on assay that precisely maps the transcription start sites genome-wide with high sensitivity and specificity in ZNF143_T cells.
- Treatments
- 500 nM dTAGV-1 for 6 hours
- Nucleic acid type
- RNA
- Average fragment size
- 40 [1-1]39 [2-1]
- Size selection method
- Streptavidin magnetic beads
- Library construction method
- capped RNA enrichment
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Haiyuan Yu, Cornell
- Award
- UM1HG009393 (John Lis, Cornell)
- Project
- ENCODE
- Aliases
- haiyuan-yu:procap-experiment-ZNF143_T
- Date released
- January 16, 2026
- Submitter comment
- Experiment performed by Sagar R. Shah and preprocessed by Alden Leung and You Chen
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens HEK293T cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF143 treated with 500 nM dTAGV-1 for 6 hours | ENCBS868ZCQ | ENCGM141MGP | ENCLB911UAA |
| 2 | 1 | Homo sapiens HEK293T cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF143 treated with 500 nM dTAGV-1 for 6 hours | ENCBS997DQU | ENCGM141MGP | ENCLB152STL |