Experiment summary for ENCSR721CNY

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
DGCR8
Biosample summary
Homo sapiens K562 genetically modified using CRISPR
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against DGCR8 on day 30. (DGCR8-D30_BGKcLV02)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.6 [2-1]9.9 [1-1]
Size range
>200
Lysis method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation method
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:DGCR8-D30_BGKcLV02
Date submitted
March 15, 2016
Date released
March 23, 2016
Related datasets
ENCSR111DOM 
Time series of RNA-seq on K562 cells treated with a CRISPR gRNA against DGCR8
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv4Search for all Experiment with internal tag ENCORE

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsLibrary
21K562 cell line genetically modified (deletion) using CRISPR targeting DGCR8ENCBS091YRZENCGM467BBSENCLB194GBS
11K562 cell line genetically modified (deletion) using CRISPR targeting DGCR8ENCBS998ZJJENCGM467BBSENCLB412YOA