Experiment summary for ENCSR720HUL

Alternate accession: ENCSR531KJT

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
E2F1
Biosample summary
Homo sapiens K562
Biosample Type
cell line
Replication type
isogenic
Description
E2F1 ChIP-seq on human K562
Nucleic acid type
DNA
Size range
450-650
Strand specificity
Non-strand-specific

Attribution

ENCODE3 project
Lab
Michael Snyder, Stanford
Award
U54HG006996 (Michael Snyder, Stanford)
Project
ENCODE
External resources
Aliases
michael-snyder:ChIPss-388
Date submitted
June 30, 2015
Date released
January 26, 2018
Submitter comment
We note that this ChIP-seq experiment was performed using an antibody for which we have provided a compliant primary characterization but no secondary characterization. A secondary characterization using IP-MS was attempted, but the experiment failed. There is no remaining antibody from this lot number and therefore additional secondary characterizations cannot be attempted. However, because the replicate ChIP-seq datasets passed the ENCODE IDR ChIP-seq data quality standards, we are releasing the data to the public. This experiment was used in the ENCODE-DREAM Transcription Factor Binding Site Prediction Challenge (https://www.synapse.org/#!Synapse:syn6131484/wiki/400026). IMPORTANT: This experiment and ENCSR531KJT were both part of the DREAM challenge but have since been merged as they were the same libraries, just sequenced on two different platforms. The original analysis files have been archived. This experiment was re-processed using a control sequenced on HiSeq4000 rather than on HiSeq2000 as it was originally done so the platforms now match.
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv5Search for all Experiment with internal tag DREAM

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
21K562 cell lineENCBS207UAMENCAB357OHUENCLB524NDK
11K562 cell lineENCBS320BULENCAB357OHUENCLB549JWU