Experiment summary for ENCSR717JMX

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP594 (unc-119(tm4063) III; wgIs594) whole organism young adult 63 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing FKH-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:FKH-3-OP594-yAd
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF147GQT, ENCFF228LZG, ENCFF199UDS, ENCFF608ZMN were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP594 (unc-119(tm4063) III; wgIs594) mixed sex young adult 63 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-fkh-3ENCBS190OXEENCGM731KOLENCAB728YTOENCLB055ZVZ
11strain OP594 (unc-119(tm4063) III; wgIs594) mixed sex young adult 63 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-fkh-3ENCBS491PRGENCGM731KOLENCAB728YTOENCLB884AIU