Experiment summary for ENCSR715QWM
doi:10.17989/ENCSR715QWM
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- SDAD1
- Biosample summary
- Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens SDAD1
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on HepG2 cells treated with a CRISPR gRNA against SDAD1. (SDAD1-BGHcLV07)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.7 [2-1]9.9 [1-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:SDAD1-BGHcLV07
- Date submitted
- November 20, 2017
- Date released
- December 9, 2017
- Gene Silencing Series
- ENCSR919NYR
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SDAD1 | ENCBS045JKA | ENCGM938LKI | ENCLB954QRQ |
| 1 | 1 | Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SDAD1 | ENCBS268BDM | ENCGM938LKI | ENCLB268IKW |