Experiment summary for ENCSR714ZDK

doi:10.17989/ENCSR714ZDK

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
elt-4
Biosample summary
Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) whole organism hermaphrodite late embryo 3 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans elt-4
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing ELT-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:ELT-4-OP111-LE
Date submitted
July 29, 2015
Date released
May 24, 2016
Submitter comment
ENCFF835AUG, ENCFF008LRY, ENCFF025WVY, ENCFF863ZNH were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) hermaphrodite late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-4ENCBS585JICENCGM168PDZENCAB728YTOENCLB835SNA
21Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) hermaphrodite late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-4ENCBS008VNNENCGM168PDZENCAB728YTOENCLB760WPU