Experiment summary for ENCSR714ZDK
doi:10.17989/ENCSR714ZDK
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- elt-4
- Biosample summary
- Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) whole organism hermaphrodite late embryo 3 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans elt-4
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing ELT-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:ELT-4-OP111-LE
- Date submitted
- July 29, 2015
- Date released
- May 24, 2016
- Submitter comment
- ENCFF835AUG, ENCFF008LRY, ENCFF025WVY, ENCFF863ZNH were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) hermaphrodite late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-4 | ENCBS585JIC | ENCGM168PDZ | ENCAB728YTO | ENCLB835SNA |
2 | 1 | Caenorhabditis elegans strain OP111 (unc-119(ed3) III; wgIs111) hermaphrodite late embryo 3 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-4 | ENCBS008VNN | ENCGM168PDZ | ENCAB728YTO | ENCLB760WPU |