Experiment summary for ENCSR714ALL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
lin-14
Biosample summary
Caenorhabditis elegans strain OP768 (unc-119(tm4063); wgls768 [lin-14::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism L1 larva (6 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing LIN-14-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
valerie-reinke:LIN-14_OP768_L1
Date submitted
September 17, 2018
Date released
September 19, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11strain OP768 (unc-119(tm4063); wgls768 [lin-14::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting lin-14ENCBS317CLKENCGM642RITENCAB728YTOENCLB039UEN
21strain OP768 (unc-119(tm4063); wgls768 [lin-14::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting lin-14ENCBS016UQHENCGM642RITENCAB728YTOENCLB882OIO