Experiment summary for ENCSR698IEF
doi:10.17989/ENCSR698IEF
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Xbp1
- Biosample summary
- Drosophila melanogaster strain Xbp1-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster Xbp1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Xbp1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Xbp1-GFP
- Date submitted
- August 2, 2016
- Date released
- September 29, 2016
- Submitter comment
- ENCFF703APL, ENCFF738TLU, ENCFF703APL, ENCFF092TIF, ENCFF297TFD were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain Xbp1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Xbp1 | ENCBS612QUF | ENCGM154NTT | ENCAB728YTO | ENCLB957GSM |
| 2 | 1 | Drosophila melanogaster strain Xbp1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Xbp1 | ENCBS789ZPR | ENCGM154NTT | ENCAB728YTO | ENCLB055WUM |
| 3 | 1 | Drosophila melanogaster strain Xbp1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Xbp1 | ENCBS723FUL | ENCGM154NTT | ENCAB728YTO | ENCLB672MGI |