Experiment summary for ENCSR697MUB
doi:10.17989/ENCSR697MUB
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- elt-2
- Biosample summary
- Caenorhabditis elegans strain OP56 (unc-119(ed3) III; wgIs56) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans elt-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing ELT-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000, Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:ELT-2-OP56-L1
- Date submitted
- May 9, 2016
- Date released
- September 29, 2016
- Submitter comment
- ENCFF111ZJJ, ENCFF775UMQ, ENCFF123IDG, ENCFF097SNV were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files. In addition, this experiment and its control (ENCSR805GAD) did not have enough reads. The inputs were resequenced along with the IPs deeper, using the same libraries. The new files have been uploaded and supersede the older archived files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP56 (unc-119(ed3) III; wgIs56) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-2 | ENCBS915KXO | ENCGM676QSB | ENCAB728YTO | ENCLB844GWB |
| 1 | 1 | Caenorhabditis elegans strain OP56 (unc-119(ed3) III; wgIs56) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans elt-2 | ENCBS798RCR | ENCGM676QSB | ENCAB728YTO | ENCLB690KGV |