Experiment summary for ENCSR693VPE

doi:10.17989/ENCSR693VPE

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
unc-86
Biosample summary
Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) whole organism hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans unc-86
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing UNC-86-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:UNC-86-OP476-LE
Date submitted
May 9, 2016
Date released
September 29, 2016
Submitter comment
ENCFF048WRE, ENCFF419REI, ENCFF629VSP, ENCFF568VRT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-86ENCBS384PDKENCGM185NSYENCAB728YTOENCLB712WLP
11Caenorhabditis elegans strain OP476 (unc-119(tm4063) III; wgIs476) hermaphrodite late embryo 3.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans unc-86ENCBS582ABTENCGM185NSYENCAB728YTOENCLB657OCR