Experiment summary for ENCSR688GVV

doi:10.17989/ENCSR688GVV

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
FTO
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens FTO
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against FTO. (FTO_BGKLV36)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.8 [2-1]9.7 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:FTO_BGKLV36
Date submitted
December 12, 2016
Date released
January 4, 2017
Gene Silencing Series
ENCSR753TVZ 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens FTOENCBS258AXVENCGM377DJYENCLB795HBL
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens FTOENCBS166GHDENCGM377DJYENCLB050JFV