Experiment summary for ENCSR686FKU
doi:10.17989/ENCSR686FKU
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- zip-5
- Biosample summary
- Caenorhabditis elegans strain OP544 (unc-119(tm4063) III; wgIs544) whole organism hermaphrodite late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans zip-5
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing ZIP-5-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:ZIP-5-OP544-LE
- Date submitted
- July 29, 2015
- Date released
- May 24, 2016
- Submitter comment
- ENCFF822KPC, ENCFF712SDX, ENCFF763NBZ, ENCFF356KQH were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP544 (unc-119(tm4063) III; wgIs544) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans zip-5 | ENCBS773ZSD | ENCGM699BME | ENCAB728YTO | ENCLB913NLQ |
| 1 | 1 | Caenorhabditis elegans strain OP544 (unc-119(tm4063) III; wgIs544) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans zip-5 | ENCBS307NHL | ENCGM699BME | ENCAB728YTO | ENCLB923ZYV |