Experiment summary for ENCSR668BTV

doi:10.17989/ENCSR668BTV

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
mls-2
Biosample summary
Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) whole organism mixed stage (embryo) 2 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans mls-2
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing MLS-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:MLS-2-OP645-EM
Date submitted
October 4, 2016
Date released
October 10, 2016
Submitter comment
ENCFF328QIP, ENCFF251ZRR, ENCFF595KCQ, ENCFF088XAX were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mls-2ENCBS797YYOENCGM510LWVENCAB728YTOENCLB478OSH
11Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mls-2ENCBS372QTGENCGM510LWVENCAB728YTOENCLB976NIS