Experiment summary for ENCSR668BTV

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) whole organism mixed stage (embryo) 2 hours post synchronization using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing MLS-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:MLS-2-OP645-EM
Date submitted
October 4, 2016
Date released
October 10, 2016
Submitter comment
ENCFF328QIP, ENCFF251ZRR, ENCFF595KCQ, ENCFF088XAX were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-mls-2ENCBS797YYOENCGM510LWVENCAB728YTOENCLB478OSH
11strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-mls-2ENCBS372QTGENCGM510LWVENCAB728YTOENCLB976NIS