Experiment summary for ENCSR661BEZ
doi:10.17989/ENCSR661BEZ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- CTCF
- Biosample summary
- Drosophila melanogaster strain CTCF-GFP whole organism embryo (0-16 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster CTCF
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing CTCF-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:CTCF-GFP
- Date submitted
- December 21, 2016
- Date released
- January 11, 2017
- Submitter comment
- ENCFF633LQF, ENCFF472QZE were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 3 | 1 | Drosophila melanogaster strain CTCF-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CTCF | ENCBS986CYN | ENCGM588APF | ENCAB728YTO | ENCLB676JHA |
| 2 | 1 | Drosophila melanogaster strain CTCF-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CTCF | ENCBS366JAO | ENCGM588APF | ENCAB728YTO | ENCLB114OKL |