Experiment summary for ENCSR661BEZ

  • Status: released

Summary

Assay
ChIP-seq
Target
eGFP-CTCF
Biosample summary
Drosophila melanogaster strain CTCF-GFP whole organism embryo (0-16 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CTCF-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Platform
HiSeq 4000
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CTCF-GFP
Date submitted
December 21, 2016
Date released
January 11, 2017
Submitter comment
ENCFF633LQF, ENCFF472QZE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
31strain CTCF-GFP whole organism mixed sex embryo (0-16 hours) genetically modified (insertion) using site-specific recombination targeting eGFP-CTCFENCBS986CYNENCGM588APFENCAB728YTOENCLB676JHA
21strain CTCF-GFP whole organism mixed sex embryo (0-16 hours) genetically modified (insertion) using site-specific recombination targeting eGFP-CTCFENCBS366JAOENCGM588APFENCAB728YTOENCLB114OKL