Experiment summary for ENCSR661BEZ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
CTCF
Biosample summary
Drosophila melanogaster strain CTCF-GFP whole organism embryo (0-16 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CTCF-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CTCF-GFP
Date submitted
December 21, 2016
Date released
January 11, 2017
Submitter comment
ENCFF633LQF, ENCFF472QZE were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31strain CTCF-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting CTCFENCBS986CYNENCGM588APFENCAB728YTOENCLB676JHA
21strain CTCF-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting CTCFENCBS366JAOENCGM588APFENCAB728YTOENCLB114OKL