Experiment summary for ENCSR651PAZ
doi:10.17989/ENCSR651PAZ
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl neural tube tissue embryo (13.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 13.5 day mouse neural tube
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E13_5_NT
- Date submitted
- August 14, 2015
- Date released
- August 18, 2015
- Reference Epigenome
- ENCSR832HYG
- Organism Development Series
- ENCSR225OBZ
- Submitter comment
- Fastq files ENCFF123EEE, ENCFF697GRA, ENCFF098OFT, and ENCFF766YTP contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF123EEE (130, 200226094), (136, 418937124); ENCFF697GRA (130, 204529174), (136, 429439582); ENCFF098OFT (130, 204845320), (136, 213561768); ENCFF766YTP (130, 206988372), (136, 227227205)
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) neural tube tissue | ENCBS549OYR | ENCLB551IIN |
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) neural tube tissue | ENCBS956BQY | ENCLB805UZJ |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) neural tube tissue | ENCBS549OYR | ENCLB428LJN |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) neural tube tissue | ENCBS956BQY | ENCLB157NPV |