Experiment summary for ENCSR650OZF
doi:10.17989/ENCSR650OZF
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K27ac
- Biosample summary
- Homo sapiens dendritic cell treated with 100 ng/mL lipopolysaccharide for 30 minutes
- Biosample Type
- in vitro differentiated cells
- Replication type
- unreplicated
- Description
- ChIP-seq
- Treatments
- 100 ng/mL lipopolysaccharide (CHEBI:16412) for 30 minutes
- Nucleic acid type
- DNA
- Size range
- 150-400
- Extraction method
- TruChIP chromatin shearing reagent kit Covaris cat# 520154, sonication Covaris M220, IP (home protocol), DNA isolation with QIAquick PCR Purification Kit cat# 28104.
- Platform
- Illumina NextSeq 500
Attribution
Genomics of Gene Regulation Project
- Lab
- Manuel Garber, UMass
- Award
- U01HG007910 (Jeremy Luban, UMass)
- Project
- GGR
- External resources
- Aliases
- manuel-garber:F33_MDDC_H3K27ac_Lps_30m_ChIP-seq
- Date submitted
- January 17, 2017
- Date released
- January 19, 2017
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
---|---|---|---|---|---|
1 | 1 | Homo sapiens dendritic in vitro differentiated cells treated with 100 ng/mL lipopolysaccharide for 30 minutes | ENCBS550GVG | ENCAB003HJF | ENCLB950CBR |