Experiment summary for ENCSR650OZF

Summary

Status
released
Assay
ChIP-seq
Target
H3K27ac
Biosample summary
Homo sapiens dendritic cell treated with 100 ng/mL lipopolysaccharide for 30 minutes
Biosample Type
in vitro differentiated cells
Replication type
unreplicated
Description
ChIP-seq
Treatments
100 ng/mL lipopolysaccharide (CHEBI:16412) for 30 minute
Nucleic acid type
DNA
Size range
150-400
Extraction method
TruChIP chromatin shearing reagent kit Covaris cat# 520154, sonication Covaris M220, IP (home protocol), DNA isolation with QIAquick PCR Purification Kit cat# 28104.
Strand specificity
Non-strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Manuel Garber, UMass
Award
U01HG007910 (Jeremy Luban, UMass)
Project
GGR
External resources
Aliases
manuel-garber:F33_MDDC_H3K27ac_Lps_30m_ChIP-seq
Date submitted
January 17, 2017
Date released
January 19, 2017

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleAntibodyLibrary
11dendritic in vitro differentiated cells treated with 100 ng/mL lipopolysaccharide for 30 minutesENCBS550GVGENCAB003HJFENCLB950CBR