Experiment summary for ENCSR641VZA
doi:10.17989/ENCSR641VZA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- sem-2
- Biosample summary
- Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism mixed stage (embryo) (1 hour) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans sem-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing sem-2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:SEM-2_OP701_EE
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (1 hour) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sem-2 | ENCBS582GWI | ENCGM880AMQ | ENCAB728YTO | ENCLB910LMW |
3 | 1 | Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (1 hour) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sem-2 | ENCBS036EXP | ENCGM880AMQ | ENCAB728YTO | ENCLB719WZU |