Experiment summary for ENCSR640OHN

doi:10.17989/ENCSR640OHN

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Drosophila melanogaster Kc167 treated with 1 μM 20-hydroxyecdysone for 24 hours
Biosample Type
cell line
Replication type
unreplicated
Description
RNA-seq on ribo-depleted total RNA from Kc167
Treatments
1 μM 20-hydroxyecdysone (CHEBI:16587) for 24 hours
Nucleic acid type
RNA
Depleted in
rRNA
Size range
>200
Extraction method
Trizol
Fragmentation methods
chemical (generic)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR130DDI

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U54HG006994 (Susan Celniker, LBNL)
Project
modENCODE
Aliases
brenton-graveley:E-BS585
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Drosophila melanogaster Kc167 cell line treated with 1 μM 20-hydroxyecdysone for 24 hoursENCBS135ZQFENCLB546XWD