Experiment summary for ENCSR632DQP
doi:10.17989/ENCSR632DQP
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Control type
- control
- Biosample summary
- Homo sapiens A549
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 0 hours.
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 1µg
- Size range
- >200
- Extraction method
- RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
- Size selection method
- dual SPRI
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:t00_rna_seq
- Date submitted
- March 3, 2016
- Date released
- May 5, 2016
- Treatment Time Series
- ENCSR897XFT
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens A549 cell line | ENCBS797SEZ | ENCLB538FEL |
| 2 | 1 | Homo sapiens A549 cell line | ENCBS302GLU | ENCLB624CWM |
| 4 | 1 | Homo sapiens A549 cell line | ENCBS951SRH | ENCLB296PKA |
| 3 | 1 | Homo sapiens A549 cell line | ENCBS153BPN | ENCLB510CFF |