Experiment summary for ENCSR619GAS

doi:10.17989/ENCSR619GAS

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens K562 genetically modified (deletion) using CRISPR
Biosample Type
cell line
Replication type
isogenic
Description
Control CRISPR gRNA against no target in K562 cells followed by RNA-seq on day 41. (NT-D41_BGKcLV02)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10 [2-1]9.8 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NT-D41_BGKcLV02
Date submitted
March 15, 2016
Date released
March 23, 2016
Gene Silencing Series
ENCSR902LPM 
Treatment Time Series
ENCSR111DOM 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line genetically modified (deletion) using CRISPRENCBS345PNAENCGM474NKSENCLB769OJJ
11Homo sapiens K562 cell line genetically modified (deletion) using CRISPRENCBS411BWTENCGM474NKSENCLB294UXD