1. Experiments
  2. RNA-seq
  3. Mus musculus
  4. bone marrow-derived macrophage

Experiment summary for ENCSR614KOV

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Mus musculus C57BL/6 bone marrow-derived macrophage originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutes
Biosample Type
primary cell
Replication type
isogenic
Description
mRNA-seq on whole cell extract RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for480 minutes.
Treatments
100 ng/mL lipoprotein A for 480 minute
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
250-400
Extraction method
see document
Fragmentation method
see document
Size selection method
SPRI beads
Strand specificity
Non-strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
Aliases
alexander-hoffmann:PolyA.Naive.480.exp
Date submitted
April 5, 2018
Date released
November 20, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
31strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS065RKXENCLB860IBY
11strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS743ZFOENCLB378GJX
21strain C57BL/6J bone marrow-derived macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS416HYCENCLB904AIY