Experiment summary for ENCSR614KOV

doi:10.17989/ENCSR614KOV

Summary

Status
released
Assay
polyA plus RNA-seq
Biosample summary
Mus musculus strain C57BL/6J macrophage originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutes
Biosample Type
primary cell
Replication type
isogenic
Description
mRNA-seq on whole cell extract RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for480 minutes.
Treatments
100 ng/mL lipoprotein A for 480 minutes
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
250-400
Fragmentation methods
see document
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
External resources
Aliases
alexander-hoffmann:PolyA.Naive.480.exp
Date submitted
April 5, 2018
Date released
November 20, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
31Mus musculus strain C57BL/6J macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS065RKXENCLB860IBY
11Mus musculus strain C57BL/6J macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS743ZFOENCLB378GJX
21Mus musculus strain C57BL/6J macrophage primary cell originated from bone marrow treated with 100 ng/mL lipoprotein A for 480 minutesENCBS416HYCENCLB904AIY