Experiment summary for ENCSR603LBA

doi:10.17989/ENCSR603LBA

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Sp1
Biosample summary
Drosophila melanogaster strain Sp1-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sp1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing -eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Sp1-GFP
Date submitted
April 12, 2019
Date released
May 16, 2019

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain Sp1-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sp1ENCBS027NWSENCGM537ZDZENCAB728YTOENCLB236FRY
11Drosophila melanogaster strain Sp1-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sp1ENCBS002MHHENCGM537ZDZENCAB728YTOENCLB690MOW
21Drosophila melanogaster strain Sp1-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sp1ENCBS290RSJENCGM537ZDZENCAB728YTOENCLB012DXA