Experiment summary for ENCSR596SMN
doi:10.17989/ENCSR596SMN
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Mus musculus strain C57BL/6J macrophage nuclear fraction
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- Ribo depletion RNA-seq on nucleoplasmic fraction RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for 60 minute.
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Library starting quantity
- 1µg [1-1]500ng [2-1]
- Size range
- 250-400
- Fragmentation methods
- see document
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2000, Illumina HiSeq 4000
Attribution
Genomics of Gene Regulation Project
- Lab
- Alexander Hoffmann, UCLA
- Award
- U01HG007912 (Alexander Hoffmann, UCLA)
- Project
- GGR
- External resources
- Aliases
- alexander-hoffmann:Nuc.Naive.060.exp
- Date submitted
- January 20, 2017
- Date released
- November 20, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6J macrophage primary cell nuclear fraction | ENCBS616DMC | ENCLB629MFV |
| 2 | 1 | Mus musculus strain C57BL/6J macrophage primary cell nuclear fraction | ENCBS616WLF | ENCLB743QSR |