Experiment summary for ENCSR596ACL

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Homo sapiens K562 membrane fraction
Biosample Type
cell line
Replication type
isogenic
Description
Initial membrane fractions on K562 Long Poly-A+ from Lecuyer
Nucleic acid type
polyadenylated mRNA
Size range
>200
Lysis method
Trizol (LifeTech cat#: 15596-018)
Extraction method
Trizol (LifeTech cat#: 15596-018)
Fragmentation method
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific

Attribution

ENCODE3 project
Lab
Eric Lécuyer, IRCM
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
eric-lecuyer:K562-initPolyA-Membrane
Date submitted
February 1, 2016
Date released
April 21, 2016
Tags
Search for all Experiment with internal tag ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11K562 cell line membrane fractionENCBS949ESXENCLB460RTX
21K562 cell line membrane fractionENCBS985INMENCLB486WAD