Experiment summary for ENCSR588TIV

doi:10.17989/ENCSR588TIV

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Homo sapiens T-helper 2 cell male adult (35 years) treated with 100 ng/mL Interleukin-4, 5 μg/mL Interferon-gamma antibody
Biosample Type
primary cell
Replication type
isogenic
Description
CD4 N (RA+ MACS sort)Th2 (aIFNg 5ug/ml+IL-4 100ng/ml)(day 14)
Treatments
100 ng/mL Interleukin-4 (UniProtKB:P05112) , 5 μg/mL Interferon-gamma antibody
Nucleic acid type
RNA
RNA integrity number
9
Library starting quantity
300ng
Average fragment size
224 [1-1]277 [2-1]
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR597UQL

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Date released
July 14, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (35 years) T-helper 2 primary cell treated with 100 ng/mL Interleukin-4, 5 μg/mL Interferon-gamma antibodyENCBS983OVZENCLB977MZE
21Homo sapiens male adult (35 years) T-helper 2 primary cell treated with 100 ng/mL Interleukin-4, 5 μg/mL Interferon-gamma antibodyENCBS727FRTENCLB333MSB