Experiment summary for ENCSR584UYK

doi:10.17989/ENCSR584UYK

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens HepG2 expressing RNAi
Biosample Type
cell line
Replication type
isogenic
Description
Control shRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHLV37-B)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10 [1-1]9.6 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NT-BGHLV37-B
Date submitted
November 20, 2017
Date released
December 6, 2017
Gene Silencing Series
ENCSR102PLQ 
, ENCSR714IVS 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAiENCBS496TVIENCGM266AWPENCLB246EQH
21Homo sapiens HepG2 cell line expressing RNAiENCBS135CDEENCGM266AWPENCLB589ZPI